AB157. Treatment of diabetic erectile dysfunction by using endothelial progenitor cells genetically modified by human telomerase reverse transcriptase
نویسندگان
چکیده
Objective: PDE5 inhibitors represent the first line therapy for treatment of ED. However, diabetic patients have poorer response compared with normal patients. The aim of this study was to determine whether taurine, a sulfurcontaining amino acid, affects diabetic erectile dysfunction. Methods: Type 1 diabetes mellitus was induced in male rats by streptozotocin (60 mg/kg, intraperitoneally). After 12 weeks, apomorphine test was conducted to confirm diabetic erectile dysfunction (DED). Only DED rats were administered taurine (400 mg/kg/day, intraperitoneally) or vehicle, for 4 weeks. Age-matched control, nondiabetic, rats were treated with saline intraperitoneally for 4 weeks. At week 16, after a 2-day washout, erectile function was evaluated. Peniles were harvested for Western blot analysis of RhoA, ROCK-1, ROCK-2, eNOS, nNOS, NADPH oxidase subunit gp91phox. Results: Erectile function was significantly destroyed in diabetic rats compared with nondiabetic rats and was ameliorated in diabetic rats treated with taurine. In diabetic rats, RhoA, ROCK-1, ROCK-2 and gp91phox protein expressions were increased, whereas eNOS and nNOS expressions were decreased compared with nondiabetic rats, and both were reversed in diabetic rats treated with taurine. Conclusions: Taurine improves erectile function in diabetic rats probably by inhibiting RhoA/Rock and activating NOS/NO signaling pathways. This may provide a potential new therapy for diabetic ED.
منابع مشابه
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase
The efficacy of treatments for diabetes mellitus-induced erectile dysfunction (DMED) is quite poor, and stem cell therapy is emerging as a useful method. In this study, we used endothelial progenitor cells (EPCs) overexpressing human telomerase reverse transcriptase (hTERT) for the treatment of DMED. Rat EPCs were transfected with hTERT (EPCs-hTERT). EPCs-hTERT secreted more growth factors and ...
متن کاملChronic Exposure of Human Endothelial Progenitor Cells to Diabetic Condition Abolished the Regulated Kinetics Activity of Exosomes
By virtue of lifestyle change, incidence of type 2 diabetes is increasingly being raised with different up-surging pathologies. This condition found to disqualify endothelial progenitor cells during neo-vascularization. Besides to an aborted differentiation property, malfunctioned paracrine activities exacerbate vascular abnormalities. It is found nano-scaled exosomes play essential roles on re...
متن کاملExpression Pattern of Alternative Splicing Variants of Human Telomerase Reverse Transcriptase (hTERT) in Cancer Cell Lines Was not Associated with the Origin of the Cells
Telomerase and systems controlling their activity have been of great attention. There are controversies regarding the role of the alternative splicing forms of the human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase. Therefore, the correlation between telomerase enzyme activity, the abundance of alternatively spliced variants of hTERT and doubling time of a seri...
متن کاملChronic Exposure of Human Endothelial Progenitor Cells to Diabetic Condition Abolished the Regulated Kinetics Activity of Exosomes
By virtue of lifestyle change, incidence of type 2 diabetes is increasingly being raised with different up-surging pathologies. This condition found to disqualify endothelial progenitor cells during neo-vascularization. Besides to an aborted differentiation property, malfunctioned paracrine activities exacerbate vascular abnormalities. It is found nano-scaled exosomes play essential roles on re...
متن کاملHuman Urine-Derived Stem Cells Alone or Genetically-Modified with FGF2 Improve Type 2 Diabetic Erectile Dysfunction in a Rat Model
AIM The aim of this study was to determine the possibility of improving erectile dysfunction using cell therapy with either human urine-derived stem cells (USCs) or USCs genetically-modified with FGF2 in a type 2 diabetic rat model. METHODS Human USCs were collected from 3 healthy donors. USCs were transfected with FGF2 (USCs-FGF2). Sixty-five SD male rats were divided into five groups (G). A...
متن کامل